Pretreatment is considered to be the heart of the wet processing of cotton. As an integral part of cotton wet processing, the scouring & bleaching process is usually named as pretreatment. Because of inherited characteristics of comfortability, absorbency, breathability, etc., cotton fabrics are still on the top in the list of people’s demands.The objective of the study is to analysis the effect of chemicals on different physical properties and color fastness of treated cotton samples with standard rating values.